Journal: JCI Insight
Article Title: COVID-19 and RA share an SPP1 myeloid pathway that drives PD-L1 + neutrophils and CD14 + monocytes
doi: 10.1172/jci.insight.147413
Figure Lengend Snippet: ( A ) Patients and healthy donors, shown as the following: n = 121 patients with acute pneumonia ( n = 29 community acquired SARS-CoV-2 – pneumonia, n = 29 mild/moderate COVID-19, n = 63 severe COVID-19), convalescent COVID-19 ( n = 41), and healthy controls ( n = 10). Representative images of lung CT scans. ( B ) Plasma levels of SPP1, S100A12, GAS6, and PROS1 in groups as in A . ( C ) Spearman’s rank correlations between SPP1, S100A12, GAS6, and PROS1 plasma levels in patients with acute COVID-19 pneumonia ( n = 92) with demographic and clinical parameters. Each box displays the r value, and an asterisk indicates statistical significance of P < 0.05. ( D ) Plasma levels of SPP1, S100A12, GAS6, and PROS1 in patients with acute COVID-19 pneumonia ( n = 92) stratified based on lung functions measured by PaO 2 /FiO 2 at the time of hospital admission. Severe respiratory failure was defined by PaO 2 /FiO 2 ≤ 200. ( E ) Percentage of acute COVID-19 pneumonia patients ( n = 92) with PaO 2 /FiO 2 ≤ 200 based on high plasma levels of SPP1 (≥108 ng/mL), S100A12 (≥59 ng/mL), GAS6 (≥24 ng/mL), and PROS1 (≥15 μg/mL). ( F ) COVID-19 patient plasma levels of SPP1, S100A12, GAS6, and PROS1 at the time of hospital admission ( n = 92) stratified based on a patient’s subsequent need to be transferred to ICU. ( G ) Percentage of patients with acute COVID-19 pneumonia ( n = 92) transferred to ICU during the hospitalization based on having high levels of SPP1 (≥108 ng/mL), S100A12 (≥59 ng/mL), GAS6 (≥24 ng/mL), and PROS1 (≥15 μg/mL) at the time of hospital admission. ( B , D , and F ) Data are presented as violin plots with median and interquartile range. Asterisk indicates 1-way ANOVA (Kruskal-Wallis test) with Dunn’s correction for multiple comparisons if more than 2 groups were compared ( B ), or 2-sided Mann-Whitney U was used when 2 groups were compared ( B and D – G ). ( H ) Kaplan-Meier analysis of the rate of transfer of COVID-19 patients to ICU based on their cut-off values for SPP1, S100A12, GAS6, and PROS1 at the time of hospital admission.
Article Snippet: Blood samples were centrifuged (600 g /15 minutes) and plasma aliquots were stored at –80°C until analysis by ELISA for SPP1 (BMS2066; Thermo Fisher Scientific), S100A12 (DY1052; R&D Systems), GAS6 (DY885B; R&D Systems), and PROS1 (NBP2-60585; NOVUS Biological).
Techniques: Clinical Proteomics, MANN-WHITNEY
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